Publications

Synthetically programmed antioxidant delivery by a domesticated skin commensal

Guillermo Nevot, Javier Santos‐Moreno, Nil Campamà Sanz, Lorena Toloza, Cristóbal Parra-Cid, Patrick A. M. Jansen, Içvara Barbier, Rodrigo Ledesma-Amaro, Ellen H. Van Den Bogaard, Marc Güell

Synthetic biology of Fusarium for the sustainable production of valuable bioproducts

Rima Gnaim, Rodrigo Ledesma-Amaro

Staying productive under pressure: systems evaluations of β-carotene production in Yarrowia lipolytica under continuous fermentation

Alyssa M. Worland, Vincent Xu, Marcela Durán, Philip Gitman, Kristen Hunter-Cevera, Cinzia Klemm, Yufei Sun, Diego Ruiz Sanchis, Rodrigo Ledesma-Amaro, Kyle Pomraning, Deepti Tanjore, Mark Blenner, Yinjie Tang

Rhodotorula Yeasts as Potential Chassis for Sustainable Food Biotechnology

Xiaochun Zheng, Rodrigo Ledesma-Amaro, Zongbao K. Zhao, Y. Wang, Xin‐Qing Zhao, Xiaobing Yang

PromoterAtlas: decoding regulatory sequences across Gammaproteobacteria using a transformer model

Lucas Coppens, Rodrigo Ledesma-Amaro

Pioneering microbial synthesis of gangliosides in the filamentous fungus Ashbya gossypii

Javier F. Montero-Bullón, Javier Martín‐González, Rodrigo Ledesma-Amaro, Alberto Jiménez, Rubén M. Buey

Microalgae-based vaccines for aquaculture

Houcong Chen, Jian‐Guo Jiang, Rodrigo Ledesma-Amaro

Metabolic engineering of Yarrowia lipolytica for the production and secretion of the saffron ingredient crocetin

Tingan Zhou, Young-Kyoung Park, Jing Fu, Piotr Hapeta, Cinzia Klemm, Rodrigo Ledesma-Amaro

Metabolic engineering of Yarrowia lipolytica for efficient conversion of valorised food waste into D-lactic acid

T. Chandukishore, Korrapati Narasimhulu, Rodrigo Ledesma-Amaro, Ashish A. Prabhu

Metabolic Engineering of Rhodotorula toruloides for the Production of Linalool

Xiaochun Zheng, Yajun Li, Chun‐Tao Che, Qidou Gao, Xue Wang, Rodrigo Ledesma-Amaro, Zi Wei Luo, Xin Lü, Y. Wang, Xiaobing Yang

Investigating the Potential of Division of Labor in Synthetic Bacterial Communities for the Production of Violacein

Harman Mehta, José I. Jiménez, Rodrigo Ledesma-Amaro, Guy‐Bart Stan

Insights into the methanol utilization capacity of Y. lipolytica and improvements through metabolic engineering

Wei Jiang, William Newell, Jingjing Liu, Lucas Coppens, Khushboo Borah, Huadong Peng, David Bell, Long Liu, Victoria S. Haritos, Rodrigo Ledesma-Amaro

Genome-based identification, and characterization of a bacteriocin selective inhibiting Penicillium citrinum in yak yogurt

Yongqi Yang, Huojian Zheng, Cancan Qi, Qin Wang, Rodrigo Ledesma-Amaro, Liping Wang

Fusarium-based mycoprotein: Advancements in the production of sustainable meat substitutes

Rima Gnaim, Paul S. Dyer, Rodrigo Ledesma-Amaro

From Circuits to Symphonies: Redefining Synthetic Biology for Multimicrobial Systems

Miguel Fernández-Niño, Ludger A. Wessjohann, José Manuel Guillamón, Daniela Burgos-Toro, Silvia Moriano‐Gutierrez, Giacomo Di Matteo, Ziaul Islam, Rinke J. Van Tatenhove‐Pel, Vanessa R. Marcelino, Rodrigo Ledesma-Amaro

Exploring the Fermentation Potential of Kluyveromyces marxianus NS127 for Single-Cell Protein Production

Lichao Dong, Wu Yanyan, Mingxia Li, Chan Zhang, Jialu Cao, Rodrigo Ledesma-Amaro, Weiwei Zhao, Dingrong Kang

Enhancing Precursor Supply and Engineering Efflux Systems to Improve Abscisic Acid Production and Secretion in Yarrowia lipolytica

Mei-Li Sun, Yun Xu, Lu Lin, Jian Gao, Rodrigo Ledesma-Amaro, Kaifeng Wang, Xiao‐Jun Ji

Engineering strategies for producing medium-long chain dicarboxylic acids in oleaginous yeasts

Xin Wang, Mei-Li Sun, Lu Lin, Rodrigo Ledesma-Amaro, Kaifeng Wang, Xiao‐Jun Ji

Engineered Membraneless Organelles in Corynebacterium Glutamicum For Enhanced Indigoidine Biosynthesis and Antimicrobial Peptide Production

Manman Sun, Yimeng Zhao, Xiong Gao, Xiuxia Liu, Rodrigo Ledesma-Amaro, Zhonghu Bai, Peng Wang

Engineered Membraneless Organelles in Corynebacterium glutamicum for Enhanced Indigoidine Biosynthesis and Antimicrobial Peptide Production

Manman Sun, Y. -C. Zhao, Rodrigo Ledesma-Amaro, Jin Gao, Xiuxia Liu, Zhonghu Bai, Alex Xiong Gao, Peng Wang

Efficient biosynthesis of β-caryophyllene by engineered Yarrowia lipolytica

Young-Kyoung Park, Lucie Studená, Piotr Hapeta, Ramdane Haddouche, David Bell, Pablo Torres-Montero, José L. Martínez, Jean‐Marc Nicaud, A. Botes, Rodrigo Ledesma-Amaro

Dynamic regulation combined with systematic metabolic engineering for high-level palmitoleic acid accumulation in oleaginous yeast

Yufan Zhou, Mei-Li Sun, Lu Lin, Rodrigo Ledesma-Amaro, Kaifeng Wang, Xiao‐Jun Ji, He Huang

CRISPR/Cas13X-assisted programmable and multiplexed translation regulation for controlled biosynthesis

X. Xu, Xueqin Lv, Yanfeng Liu, Jianghua Li, Guocheng Du, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production in Yarrowia lipolytica

Tao Sun, Mei-Li Sun, Lu Lin, Jian Gao, Rodrigo Ledesma-Amaro, Kaifeng Wang, Xiao‐Jun Ji

Biosynthetic Pathway, Structural Characterization and Antioxidant Activity of Lactobacillus Fermentum Sn4-1 Extracellular Polysaccharide in Yak Yogurt

Wenqi Zou, Yongqi Yang, Fan Ying, Rodrigo Ledesma-Amaro, Qin Wang, Liping Wang

Biosynthetic Pathway, Structural Characterization and Antioxidant Activity of Lactobacillus Fermentum Sn4-1 Extracellular Polysaccharide in Yak Yogurt

Wenqi Zou, Yongqi Yang, Ying Fan, Rodrigo Ledesma-Amaro, Qin Wang, Liping Wang

Advances in the biosynthesis of β-carotene and its derivatives in yeast

Tingan Zhou, Young-Kyoung Park, Rodrigo Ledesma-Amaro

Advancements and Challenges in the Bioproduction of Raspberry Ketone by Precision Fermentation

Pin Gao, Haiqing Sun, Rodrigo Ledesma-Amaro, Esteban Marcellin, Huadong Peng

Understanding resource competition to achieve predictable synthetic gene expression in eukaryotes

Roberto Di Blasi, Jacopo Gabrielli, Kiyan Shabestary, Ioanna Ziarti, Tom Ellis, Cleo Kontoravdi, Rodrigo Ledesma-Amaro, Francesca Ceroni

Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid

Mei-Li Sun, Ziyun Zou, Lu Lin, Rodrigo Ledesma-Amaro, Kaifeng Wang, Xiao‐Jun Ji

Synthetic, marine, light-driven, autotroph-heterotroph co-culture system for sustainable β-caryophyllene production

Wenchao Chen, Young-Kyoung Park, Lucie Studená, David Bell, Piotr Hapeta, Jing Fu, Peter J. Nixon, Rodrigo Ledesma-Amaro

Reprogramming Yarrowia lipolytica metabolism for efficient synthesis of itaconic acid from flask to semipilot scale

Jing Fu, Simone Zaghen, Hongzhong Lu, Oliver Konzock, Naghmeh Poorinmohammad, A Kornberg, Rodrigo Ledesma-Amaro, Deni Košeto, Alexander Wentzel, Francesca Di Bartolomeo, Eduard J. Kerkhoven

Phenotypic heterogeneity follows a growth-viability tradeoff in response to amino acid identity

Kiyan Shabestary, Cinzia Klemm, Benedict Carling, James Marshall, Juline Savigny, Marko Storch, Rodrigo Ledesma-Amaro

Multiplexed in-situ mutagenesis driven by a dCas12a-based dual-function base editor

Yaokang Wu, Yang Li, Yanfeng Liu, Xiang Xiu, Jiaheng Liu, Linpei Zhang, Jianghua Li, Guocheng Du, Xueqin Lv, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

Microbial conversion of ethanol to high-value products: progress and challenges

Manman Sun, Alex Xiong Gao, Xiuxia Liu, Zhonghu Bai, Peng Wang, Rodrigo Ledesma-Amaro

Hyper‐production of porcine contagious pleuropneumonia subunit vaccine proteins in Escherichia coli by developing a bicistronic T7 expression system

Manman Sun, Alex Xiong Gao, Li An, Rodrigo Ledesma-Amaro, Peng Wang, Wenchao Chen, Zhonghu Bai, Xiuxia Liu

Harnessing oleaginous yeast to produce omega fatty acids

Lu Lin, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji, He Huang

Enhancing Plant Photosynthesis using Carbon Dots as Light Converter and Photosensitizer

Haitao Hu, Wenbo Cheng, Xueyun Wang, Yang Yu, Xuemeng Yu, Jianwei Ding, Yiliang Lin, Wei Zhao, Qian Zhao, Rodrigo Ledesma-Amaro, Xihan Chen, Junzhong Liu, Yang Chen, Xiang Gao

Engineering Yarrowia lipolytica for Sustainable Production of the Pomegranate Seed Oil-Derived Punicic Acid

Kaifeng Wang, Yueyue Zhou, Lizhen Cao, Lu Lin, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Engineering Yarrowia lipolytica for Efficient Synthesis of Geranylgeraniol

Kaifeng Wang, Mingxue Yin, Mei-Li Sun, Quanyu Zhao, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji, Lu Lin

Engineered cross-feeding creates inter- and intra-species synthetic yeast communities with enhanced bioproduction

Young-Kyoung Park, Huadong Peng, Piotr Hapeta, Lara Sellés Vidal, Rodrigo Ledesma-Amaro

Designing synthetic microbial communities with the capacity to upcycle fermentation byproducts to increase production yields

Razieh Rafieenia, Cinzia Klemm, Piotr Hapeta, Jing Fu, M. García, Rodrigo Ledesma-Amaro

DELLA proteins recruit the Mediator complex subunit MED15 to coactivate transcription in land plants

Jorge Hernández‐García, Antonio Serrano-Mislata, María Lozano-Quiles, Cristina Úrbez, María A. Nohales, Noel Blanco‐Touriñán, Huadong Peng, Rodrigo Ledesma-Amaro, Miguel Á. Blázquez

De novo engineering of programmable and multi-functional biomolecular condensates for controlled biosynthesis

Wenwen Yu, Ke Jin, Dandan Wang, Nan‐Kai Wang, Yangyang Li, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

Constructing a green oleaginous yeast cell factory for sustainable production of the plant-derived diterpenoid sclareol

Mei-Li Sun, Yuting Han, Xiao Yu, Kaifeng Wang, Lu Lin, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities

Huadong Peng, Alexander P. S. Darlington, Eric J. South, Hao‐Hong Chen, Wei Jiang, Rodrigo Ledesma-Amaro

What is the key challenge in engineering microbiomes?

Ophelia S. Venturelli, Harris H. Wang, Sylvie Estrela, Kerwyn Casey Huang, Rodrigo Ledesma-Amaro, Alex J. H. Fedorec, Bärbel Stecher, Christopher E. Lawson, Amir Zarrinpar, Chun‐Jun Guo, Orkun S. Soyer

Vibrio natriegens genome‐scale modeling reveals insights into halophilic adaptations and resource allocation

Lucas Coppens, Tanya Tschirhart, Dagmar H. Leary, Sophie M. Colston, Jaimee R. Compton, William Judson Hervey, Karl L Dana, Gary J. Vora, Sergio Bordel, Rodrigo Ledesma-Amaro

The microbial food revolution

Alicia E. Graham, Rodrigo Ledesma-Amaro

Synthetic biology tools for environmental protection

Javad Aminian-Dehkordi, Shadi Rahimi, Mehdi Golzar-Ahmadi, Amritpal Singh, Javiera López, Rodrigo Ledesma-Amaro, Ivan Mijaković

Studies on the Anticancer and Antioxidant Activities of Resveratrol and Long-Chain Fatty Acid Esters

Patrycja Szczepańska, Magdalena Rychlicka, Sylwia Groborz, Angelika Kruszyńska, Rodrigo Ledesma-Amaro, Andrzej Rapak, Anna Gliszczyńska, Zbigniew Lazar

Spontaneously established syntrophic yeast communities improve bioproduction

Simran Kaur Aulakh, Lara Sellés Vidal, Eric J. South, Huadong Peng, Sreejith J. Varma, Lucía Herrera-Domínguez, Markus Ralser, Rodrigo Ledesma-Amaro

Reprogramming the fatty acid metabolism of Yarrowia lipolytica to produce the customized omega-6 polyunsaturated fatty acids

Jinpeng Wang, Xiao Yu, Kaifeng Wang, Lu Lin, Huhu Liu, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Remodeling Isoprene Pyrophosphate Metabolism for Promoting Terpenoids Bioproduction

X. Xu, Xueqin Lv, Shixiu Cui, Yanfeng Liu, Hongzhi Xia, Jianghua Li, Guocheng Du, Zhaofeng Li, Rodrigo Ledesma-Amaro, Jian Chen, Long Liu

Protein engineering in the computational age: An open source framework for exploring mutational landscapes in silico

Shirin Bamezai, Giovanni Maresca di Serracapriola, Freya Morris, Rasmus Hildebrandt, Marc Augustine Sojerido Amil, Rodrigo Ledesma-Amaro

Precise in-field molecular diagnostics of crop diseases by smartphone-based mutation-resolved pathogenic RNA analysis

Ting Zhang, Qingdong Zeng, Fan Ji, Honghong Wu, Rodrigo Ledesma-Amaro, Qingshan Wei, Hao Yang, Xuhan Xia, Yao Ren, Keqing Mu, Qiang He, Zhensheng Kang, Ruijie Deng

Modular Metabolic Engineering and Synthetic Coculture Strategies for the Production of Aromatic Compounds in Yeast

Huadong Peng, Ruiqi Chen, W. M. Shaw, Piotr Hapeta, Wei Jiang, David Bell, Tom Ellis, Rodrigo Ledesma-Amaro

Machine learning in biohydrogen production: a review

Avinash Alagumalai, D. Balaji, Hua Song, Somchai Wongwises, Rodrigo Ledesma-Amaro, Omid Mahian, Mikhail А. Sheremet, Éric Lichtfouse

Leaderless Bicistronic Design for Precise and Reliable Control of Gene Expression in Corynebacterium Glutamicum

Xiuxia Liu, Manman Sun, Alex Xiong Gao, Rodrigo Ledesma-Amaro, Qiuwu Fang, Yankun Yang, Zhonghu Bai

Hyper-production of porcine contagious pleuropneumonia subunit vaccine proteins in Escherichia coli by developing a bicistronic T7 expression system

Manman Sun, Xiong Gao, An Li, Rodrigo Ledesma-Amaro, Zhonghu Bai, Yankun Yang, Xiuxia Liu

Host-aware RNA-based control of microbial consortia

Alice Boo, Harman Mehta, Rodrigo Ledesma-Amaro, Guy‐Bart Stan

Highly efficient neutralizer-free l-malic acid production using engineered Saccharomyces cerevisiae

Li Sun, Quanwei Zhang, Xiao Kong, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Rodrigo Ledesma-Amaro, Jian Chen, Long Liu

High-throughput process development from gene cloning to protein production

Manman Sun, Alex Xiong Gao, Xiuxia Liu, Yankun Yang, Rodrigo Ledesma-Amaro, Zhonghu Bai

Fluorescent Probe Combined with Photoelectric Analysis Technology for Detection of Escherichia coli

Qian Cui, Yongjie Zhong, Wenkai Shang, Fuming Deng, Buhua Wang, Jia‐Jia Wu, Peng Wang, Liudang Wan, Keling Wang, Lingchen Fang, Rui Dai, Han Zhang, Rodrigo Ledesma-Amaro, Yunuo Zhang, Jiao‐Mei Huang

Evaluating DFHBI-Responsive RNA Light-Up Aptamers as Fluorescent Reporters for Gene Expression

Alicia Climent-Catala, Iván Casas‐Rodrigo, Suhasini Iyer, Rodrigo Ledesma-Amaro, Thomas E. Ouldridge

etiBsu1209: A comprehensive multiscale metabolic model for Bacillus subtilis

Xinyu Bi, Yang Cheng, Xianhao Xu, Xueqin Lv, Yanfeng Liu, Jianghua Li, Guocheng Du, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

Efficient production of mycosporine-like amino acids, natural sunscreens, in Yarrowia lipolytica

Hyunbin Jin, Sojeong Kim, Daeyeol Lee, Rodrigo Ledesma-Amaro, Ji‐Sook Hahn

CRISPR–dCas12a-mediated genetic circuit cascades for multiplexed pathway optimization

Yaokang Wu, Yang Li, Ke Jin, Linpei Zhang, Jianghua Li, Yanfeng Liu, Guocheng Du, Xueqin Lv, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

CRISPR-based biosensors for pathogenic biosafety

Hao Yang, Rodrigo Ledesma-Amaro, Hong Gao, Yao Ren, Ruijie Deng

Combining orthogonal plant and non-plant fatty acid biosynthesis pathways for efficient production of microbial oil enriched in nervonic acid in Yarrowia lipolytica

Kaifeng Wang, Lu Lin, Ping Wei, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Combination of microbial and chemical synthesis for the sustainable production of β‐elemene, a promising plant‐extracted anticancer compound

Wenjuan Li, Jie Mai, Lu Lin, Zhigang Zhang, Rodrigo Ledesma-Amaro, Weiliang Dong, Xiao‐Jun Ji

Combination of a Push–Pull–Block Strategy with a Heterologous Xylose Assimilation Pathway toward Lipid Overproduction from Lignocellulose in Yarrowia lipolytica

Tao Sun, Yizi Yu, Lexin Wang, Yichun Qi, Tian Xu, Zhe Wang, Lu Lin, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Cascaded de novo biosynthesis of lacto-proteins from CO2 by engineered Pichia pastoris

Xueqin Lv, Shixiu Cui, Jie Chen, Lingling Wang, Yanfeng Liu, Jianghua Li, Guocheng Du, Xiaohao Liu, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

Building Yarrowia lipolytica Cell Factories for Advanced Biomanufacturing: Challenges and Solutions

Mei-Li Sun, Xiaoxia Gao, Lu Lin, Jing Yang, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Biotechnology and Bioengineering: Volume 120, Number 4, April 2023

Shahin Amiri, Setare Adibzadeh, Samaneh Ghanbari, Behnaz Rahmani, Mohammad Kheirandish, Aref Farokhi‐Fard, Mansoureh Shahbazi Dastjerdeh, Fatemeh Davami, Heba Sayed Mostafa, Marwa Mahmoud Hashem, N. Qin, Lingyun Li, Zheng Wang, Shuobo Shi, Toehold, Synthe Biology, Sai ., Sumanjali Yarra, Ganapathy Ashok, Utpal Mohan, Taylor Brown, Sara Alharbi, Karen J. Ho, Bin Jiang, Maja Papež, Jiménez Lancho, Peter Eisenhut, Krishna Motheramgari, Nicole Borth, Christoph Keysberg, Helga Schneider, Kerstin Otte, Hannah S. Zurier, Julie Goddard, Kaifeng Wang, Wenyang Zhao, Lu Lin, Tianjing Wang, Ping Wei, Rodrigo Ledesma-Amaro, Aihui Zhang, Xiao‐Jun Ji, Xu Wang, Jin Ma, Pengwei Li, Xiao-Man Huang, He Sun, Matthias Knödler, Patrick Opdensteinen, Ramya Ambur Sankaranarayanan, Agnieszka Morgenroth, Eva Miriam Buhl, Felix M. Mottaghy, Johannes F. Buyel, Patrick G. Negulescu, Derrick Risner, Edward S. Spang, Daniel Sumner, David E. Block, Somen Nandi, Karen A. McDonald, Young Lyun Oh, M. R. Becker, Kerri M. Mendola, Leila H. Choe, Kelvin H. Lee, Yinges Yigzaw, Alexander Seay, Jerome Bill, Xuanwen Li, David J. Roush, Steven M. Cramer, Stefano Menegatti, Abraham M. Lenhoff, Yanying Yu, Bingxue Gong, Huili Wang, Guixia Yang, Xianxuan Zhou, Kentaro Inokuma, Shunya Miyamoto, Kohei Morinaga, Yuma Kobayashi, Ryota Kumokita, Takahiro Bamba, Yoichiro Ito, Akihiko Kondo, Tomohisa Hasunuma

Biorefining of rapeseed meal: A new and sustainable strategy for improving Cr(VI) biosorption on residual wastes from agricultural byproducts after phenolic extraction

Pei Liu, Yangmin Gong, Chen Yang, Rodrigo Ledesma-Amaro, Young-Kyoung Park, Shiyu Deng, Yuliang Wang, Wei H, Wenchao Chen

Bioconversion of Glucose-Rich Lignocellulosic Wood Hydrolysates to 3-Hydroxypropionic Acid and Succinic Acid using EngineeredSaccharomyces cerevisiae

Scott Bottoms, Christina Mürk, Huadong Peng, Rodrigo Ledesma-Amaro, Mart Loog

A primer to directed evolution: current methodologies and future directions

Lara Sellés Vidal, Mark Isalan, John Heap, Rodrigo Ledesma-Amaro

A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering

Tigran v. Yuzbashev, Evgeniya Y. Yuzbasheva, O. E. Melkina, Davina Patel, Д. М. Бубнов, Heiko Dietz, Rodrigo Ledesma-Amaro

What makes Yarrowia lipolytica well suited for industry?

Young-Kyoung Park, Rodrigo Ledesma-Amaro

Unraveling the potential of non-conventional yeasts in biotechnology

Cecilia Geijer, Rodrigo Ledesma-Amaro, Elia Tomás‐Pejó

Synthetic biology and bioelectrochemical tools for electrogenetic system engineering

Joshua M. Lawrence, Yutong Yin, Paolo Bombelli, Alberto Scarampi, Marko Storch, Laura T. Wey, Alicia Climent-Catala, Geoff Baldwin, Danny O’Hare, Christopher J. Howe, Jenny Zhang, Thomas E. Ouldridge, Rodrigo Ledesma-Amaro

Resource-aware whole-cell model of division of labour in a microbial consortium for complex-substrate degradation

Eliza Atkinson, Zoltán A. Tuza, Giansimone Perrino, Guy‐Bart Stan, Rodrigo Ledesma-Amaro

Rapid screening of antimicrobial probiotics using CRISPR cascade

Yunhao Lu, Feng Li, Jinrong Bai, Rodrigo Ledesma-Amaro, Dayu Liu, Qiang He, Ruijie Deng

Production of human milk fat substitute by engineered strains of Yarrowia lipolytica

Govindprasad Bhutada, Guillaume Ménard, Rupam Kumar Bhunia, Piotr Hapeta, Rodrigo Ledesma-Amaro, Peter J. Eastmond

Principles, Tools, and Applications of Synthetic Consortia Toward Microbiome Engineering

Eliza Atkinson, Alice Boo, Huadong Peng, Guy‐Bart Stan, Rodrigo Ledesma-Amaro

Over-expression of α-bisabolene by metabolic engineering of Yarrowia lipolytica employing a golden gate DNA assembly toolbox

Walid El-Sharoud, Samar A. Zalma, Leonardo Rios‐Solis, Rodrigo Ledesma-Amaro

New synthetic biology tools for metabolic control

Xueqin Lv, Ángeles Hueso‐Gil, Xinyu Bi, Yaokang Wu, Yanfeng Liu, Long Liu, Rodrigo Ledesma-Amaro

New Insights for Biosensing: Lessons from Microbial Defense Systems

Yi Wan, Chengli Zong, Xiangpeng Li, Aimin Wang, Yan Li, Tao Yang, Qing Bao, Michael S. DuBow, Mingying Yang, Rodrigo Ledesma-Amaro, Chuanbin Mao

Metabolic and Process Engineering for Producing the Peach-Like Aroma Compound γ-Decalactone in Yarrowia lipolytica

Yizi Yu, Yufan Zhou, Kaifeng Wang, Tao Sun, Lu Lin, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Inducible expression of large gRNA arrays for multiplexed CRISPRai applications

W. M. Shaw, Lucie Studená, Kyler Roy, Piotr Hapeta, Nicholas S. McCarty, Alicia E. Graham, Tom Ellis, Rodrigo Ledesma-Amaro

Hypersecretion of OmlA antigen in Corynebacterium glutamicum through high-throughput based development process

Manman Sun, Alex Xiong Gao, Rodrigo Ledesma-Amaro, An Li, Rongbing Wang, Jianqi Nie, Zheng Pei, Yankun Yang, Zhonghu Bai, Xiuxia Liu

Genetic Manipulation of a Twin Clostridia Consortium for Co-production of n-Butanol and Isobutanol by Consolidated Bioprocessing

Zhiqiang Wen, Zhenxiang Tu, Naief Al Makishah, Dahui Fang, Rodrigo Ledesma-Amaro, Jianguo Zhu, Qi Li, Yanan Li, Mingjie Jin

Engineering Yarrowia lipolytica to Produce Tailored Chain-Length Fatty Acids and Their Derivatives

Kaifeng Wang, Tian‐Qiong Shi, Lu Lin, Ping Wei, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives

Lizhen Cao, Mingxue Yin, Tian‐Qiong Shi, Lu Lin, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Engineering the Lipid and Fatty Acid Metabolism in Yarrowia lipolytica for Sustainable Production of High Oleic Oils

Kaifeng Wang, Tian‐Qiong Shi, Jinpeng Wang, Ping Wei, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Editorial overview: Chemical Biotechnology

Valerie C. A. Ward, Rodrigo Ledesma-Amaro

Division of labor for substrate utilization in natural and synthetic microbial communities

Razieh Rafieenia, Eliza Atkinson, Rodrigo Ledesma-Amaro

Development of a dedicated Golden Gate Assembly Platform (RtGGA) for Rhodotorula toruloides

Nemailla Bonturi, Marina Julio Pinheiro, Paola Monteiro De Oliveira, Eka Rusadze, Tobias Eichinger, Gintare Liudžiūtė, Juliano Sabedotti De Biaggi, Age Brauer, Maido Remm, Everson Alves Miranda, Rodrigo Ledesma-Amaro, Petri‐Jaan Lahtvee

De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts

Yameng Xu, Xinglong Wang, Chenyang Zhang, Xuan Zhou, Xianhao Xu, Luyao Han, Xueqin Lv, Yanfeng Liu, Song Liu, Jianghua Li, Guocheng Du, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

CRISPR-Cas13a cascade-based viral RNA assay for detecting SARS-CoV-2 and its mutations in clinical samples

Di Wang, Ting Xue, Minjin Wang, Rodrigo Ledesma-Amaro, Ying Lü, Xinyue Hu, Ting Zhang, Ming Yang, Yalun Li, Xiang Jin, Ruijie Deng, Binwu Ying, Weimin Li

Combinatorial pathway engineering of Bacillus subtilis for production of structurally defined and homogeneous chitooligosaccharides

Meixi Ling, Yaokang Wu, Rongzhen Tian, Yanfeng Liu, Wenwen Yu, Guanjun Tao, Xueqin Lv, Jianghua Li, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu

Combination of a Push-Pull-Block Strategy with a Heterogonous Xylose Assimilation Pathway Toward Lipid Overproduction from Lignocellulose in Yarrowia Lipolytica

Tao Sun, Yizi Yu, Lu Lin, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Bioconversion of cellulose into bisabolene using Ruminococcus flavefaciens and Rhodosporidium toruloides

Laura E. Walls, Peter B. Otoupal, Rodrigo Ledesma-Amaro, Sharon B. Velásquez-Orta, John M. Gladden, Leonardo Rios‐Solis

Applications of synthetic yeast consortia for the production of native and non-native chemicals

Farshad Darvishi, Sajad Rafatiyan, Mohammad Hossein Abbaspour Motlagh Moghaddam, Eliza Atkinson, Rodrigo Ledesma-Amaro

Advancing Yarrowia lipolytica as a superior biomanufacturing platform by tuning gene expression using promoter engineering

Mei-Li Sun, Tian‐Qiong Shi, Lu Lin, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Advances in synthetic biology tools paving the way for the biomanufacturing of unusual fatty acids using the Yarrowia lipolytica chassis

Kaifeng Wang, Tian‐Qiong Shi, Lu Lin, Ping Wei, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji, He Huang

Advances in Metabolic Engineering Paving the Way for the Efficient Biosynthesis of Terpenes in Yeasts

Wenjuan Li, Liuwei Cui, Jie Mai, Tian‐Qiong Shi, Lu Lin, Zhigang Zhang, Rodrigo Ledesma-Amaro, Weiliang Dong, Xiao‐Jun Ji

Advances in metabolic engineering of yeasts for the production of fatty acid-derived hydrocarbon fuels

Ran Lu, Tian‐Qiong Shi, Lu Lin, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji, He Huang

A yeast‐based tool for screening mammalian diacylglycerol acyltransferase inhibitors

Peter Gajdoš, Rodrigo Ledesma-Amaro, Jean‐Marc Nicaud, Tristan Rossignol

A robust soft sensor based on artificial neural network for monitoring microbial lipid fermentation processes using Yarrowia lipolytica

Kaifeng Wang, Wenyang Zhao, Lu Lin, Tianjing Wang, Ping Wei, Rodrigo Ledesma-Amaro, Aihui Zhang, Xiao‐Jun Ji

A pathway independent multi-modular ordered control system based on thermosensors and CRISPRi improves bioproduction in Bacillus subtilis

Wenwen Yu, Ke Jin, Yaokang Wu, Quanwei Zhang, Yanfeng Liu, Jianghua Li, Guocheng Du, Jian Chen, Xueqin Lv, Rodrigo Ledesma-Amaro, Long Liu

A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution

Ting Zhang, Ruijie Deng, Yuxi Wang, Chengyong Wu, Kaixiang Zhang, Chunyan Wang, Ningqiang Gong, Rodrigo Ledesma-Amaro, Xucong Teng, Chunrong Yang, Ting Xue, Yong Zhang, Yun Hu, Qiang He, Weimin Li, Jinghong Li

Synthetic biology for future food: Research progress and future directions

Xueqin Lv, Yaokang Wu, Mengyue Gong, Jieying Deng, Yang Gu, Yanfeng Liu, Jianghua Li, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu, Jian Chen

Synergistic improvement of N-acetylglucosamine production by engineering transcription factors and balancing redox cofactors

Deng Chen, Xueqin Lv, Jianghua Li, Hongzhi Zhang, Yanfeng Liu, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu

Synergies of Systems Biology and Synthetic Biology in Human Microbiome Studies

Bouchra Ezzamouri, Saeed Shoaie, Rodrigo Ledesma-Amaro

rfaRm: An R client-side interface to facilitate the analysis of the Rfam database of RNA families

Lara Sellés Vidal, Rafael Ayala, Guy‐Bart Stan, Rodrigo Ledesma-Amaro

Reprogramming the metabolism of Klebsiella pneumoniae for efficient 1,3-propanediol production

Weijian Wang, Xiao Yu, Yongjun Wei, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Production of Long Chain Fatty Alcohols Found in Bumblebee Pheromones by Yarrowia lipolytica

Jaroslav Hambalko, Peter Gajdoš, Jean‐Marc Nicaud, Rodrigo Ledesma-Amaro, Michal Tupec, Iva Pichová, Milan Čertík

Production of human milk fat substitute by engineered strains of Yarrowia lipolytica

Govindprasad Bhutada, Guillaume Ménard, Rodrigo Ledesma-Amaro, Peter J. Eastmond

Pathway Engineering for Beta-Carotene and Carotenoid Biosynthesis in Y. lipolytica

Mauricio Pesantes-Munoz, Rodrigo Ledesma-Amaro

Multilayer Genetic Circuits for Dynamic Regulation of Metabolic Pathways

Shixiu Cui, Xueqin Lv, Xianhao Xu, Taichi Chen, Hongzhi Zhang, Yanfeng Liu, Jianghua Li, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu

Microscopy imaging of living cells in metabolic engineering

Xueqin Lv, Ke Jin, Guoyun Sun, Rodrigo Ledesma-Amaro, Long Liu

Metabolic engineering strategies to enable microbial utilization of C1 feedstocks

Wei Jiang, David Hernández Villamor, Huadong Peng, Jian Chen, Long Liu, Victoria S. Haritos, Rodrigo Ledesma-Amaro

Hypersecretion of vaccine antigen outer membrane lipoprotein A in Corynebacterium glutamicum through high-throughput based development process

Manman Sun, Xiong Gao, Rodrigo Ledesma-Amaro, An Li, Rongbing Wang, Jiangqi Nie, Zheng Pei, Yankun Yang, Zhonghu Bai, Xiuxia Liu

Engineering Yarrowia lipolytica to produce fuels and chemicals from xylose: A review

Tao Sun, Yizi Yu, Kaifeng Wang, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Engineering Yarrowia lipolytica to produce advanced biofuels: Current status and perspectives

Ran Lu, Lizhen Cao, Kaifeng Wang, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Engineering Plant Sesquiterpene Synthesis into Yeasts: A Review

Jie Mai, Wenjuan Li, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Editorial: Synthetic Biology of Yeasts for the Production of Non-Native Chemicals

Farshad Darvishi, Mark Blenner, Rodrigo Ledesma-Amaro

Editorial: Engineering Yeast to Produce Plant Natural Products

Yongjun Wei, Boyang Ji, Rodrigo Ledesma-Amaro, Tao Chen, Xiao‐Jun Ji

Control engineering and synthetic biology: working in synergy for the analysis and control of microbial systems

Giansimone Perrino, Andreas Hadjimitsis, Rodrigo Ledesma-Amaro, Guy‐Bart Stan

Comparative Genomics Analysis of Keratin-Degrading Chryseobacterium Species Reveals Their Keratinolytic Potential for Secondary Metabolite Production

Dingrong Kang, Saeed Shoaie, Samuel Jacquiod, Søren J. Sørensen, Rodrigo Ledesma-Amaro

Bioproduction of L‐piperazic acid in gram scale using Aureobasidium melanogenum

Cun-Cui Kong, Zhuangzhuang Wang, Guang-Lei Liu, Zhenming Chi, Rodrigo Ledesma-Amaro, Zhe Chi

A modular toolset for electrogenetics

Joshua M. Lawrence, Yutong Yin, Paolo Bombelli, Alberto Scarampi, Marko Storch, Laura T. Wey, A. Climent-Catala, Geoff Baldwin, Danny O’Hare, Christopher J. Howe, Jenny Zhang, Thomas E. Ouldridge, Rodrigo Ledesma-Amaro

Towards next-generation model microorganism chassis for biomanufacturing

Yanfeng Liu, Anqi Su, Jianghua Li, Rodrigo Ledesma-Amaro, Peng Xu, Guocheng Du, Long Liu

The elucidation of phosphosugar stress response in Bacillus subtilis guides strain engineering for high N‐acetylglucosamine production

Tengfei Niu, Xueqin Lv, Yanfeng Liu, Jianghua Li, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu

Synthetic metabolic channel by functional membrane microdomains for compartmentalized flux control

Xueqin Lv, Yaokang Wu, Rongzhen Tian, Yang Gu, Yanfeng Liu, Jianghua Li, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu

Recombinant β-Carotene Production by Yarrowia lipolytica – Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization

Irene Hjorth Jacobsen, Rodrigo Ledesma-Amaro, José L. Martínez

Pyruvate-responsive genetic circuits for dynamic control of central metabolism

Xianhao Xu, Xueliang Li, Yanfeng Liu, Yong-Lian Zhu, Jianghua Li, Guocheng Du, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

Nicholas S. McCarty, Alicia E. Graham, Lucie Studená, Rodrigo Ledesma-Amaro

Microbial Lipid Biotechnology to Produce Polyunsaturated Fatty Acids

Xiao‐Jun Ji, Rodrigo Ledesma-Amaro

Microbial Chassis Development for Natural Product Biosynthesis

Xianhao Xu, Yanfeng Liu, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu

Metabolic Engineering of Clostridium cellulovorans to Improve Butanol Production by Consolidated Bioprocessing

Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Mingjie Jin, Sheng Yang

Metabolic engineering for increased lipid accumulation in Yarrowia lipolytica – A Review

Jinpeng Wang, Rodrigo Ledesma-Amaro, Yongjun Wei, Boyang Ji, Xiao‐Jun Ji

Improving the homologous recombination efficiency of Yarrowia lipolytica by grafting the heterogenous component from Saccharomyces cerevisiae

Qingchun Ji, Jie Mai, Ying Ding, Yongjun Wei, Rodrigo Ledesma-Amaro, Xiao Jun Ji

Improving the homologous recombination efficiency of Yarrowia lipolytica by grafting heterologous component from Saccharomyces cerevisiae

Qingchun Ji, Jie Mai, Ying Ding, Yongjun Wei, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Evolutionary Engineering Improved d-Glucose/Xylose Cofermentation of Yarrowia lipolytica

Linlin Zhou, Zhiqiang Wen, Zedi Wang, Yuwei Zhang, Rodrigo Ledesma-Amaro, Mingjie Jin

Engineering Yarrowia lipolytica for the selective and high-level production of isocitric acid through manipulation of mitochondrial dicarboxylate–tricarboxylate carriers

Evgeniya Y. Yuzbasheva, Pasquale Scarcia, Tigran v. Yuzbashev, Eugenia Messina, Iuliia M. Kosikhina, Luigi Palmieri, Artem v. Shutov, Maria O. Taratynova, Rodrigo Ledesma-Amaro, Ferdinando Palmieri, S. P. Sineoky, Gennaro Agrimi

Engineering Yarrowia lipolytica for sustainable production of the chamomile sesquiterpene (−)-α-bisabolol

Yi-Rong Ma, Wenjuan Li, Jie Mai, Jinpeng Wang, Yongjun Wei, Rodrigo Ledesma-Amaro, Xiao‐Jun Ji

Engineering Bacterial Cellulose by Synthetic Biology

Amritpal Singh, Kenneth T. Walker, Rodrigo Ledesma-Amaro, Tom Ellis

Editorial: Synthetic Biology-Guided Metabolic Engineering

Rodrigo Ledesma-Amaro, Pablo I. Nikel, Francesca Ceroni

De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica

Qin He, Patrycja Szczepańska, Tigran v. Yuzbashev, Zbigniew Lazar, Rodrigo Ledesma-Amaro

De novo Biosynthesis of Odd-Chain Fatty Acids in Yarrowia lipolytica Enabled by Modular Pathway Engineering

Young-Kyoung Park, Rodrigo Ledesma-Amaro, Jean‐Marc Nicaud

Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community

Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Yu Jiang, Shuliang Gao, Mingjie Jin, Sheng Yang

Biovalorisation of crude glycerol and xylose into xylitol by oleaginous yeast Yarrowia lipolytica

Ashish A. Prabhu, Dominic J Thomas, Rodrigo Ledesma-Amaro, Gary A. Leeke, Ángel Medina, Carol Verheecke‐Vaessen, Frédéric Coulon, Deepti Agrawal, Vinod Kumar

Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

Ashish A. Prabhu, Rodrigo Ledesma-Amaro, Carol Sze Ki Lin, Frédéric Coulon, Vijay Kumar Thakur, Vinod Kumar

Assembly of pathway enzymes by engineering functional membrane microdomain components for improved N-acetylglucosamine synthesis in Bacillus subtilis

Xueqin Lv, Cheng Zhang, Shixiu Cui, Xianhao Xu, Lingling Wang, Jianghua Li, Guocheng Du, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

Towards semi-synthetic microbial communities: enhancing soy sauce fermentation properties in B. subtilis co-cultures

Rachatida Det-Udom, Charlie Gilbert, Long Liu, Cheunjit Prakitchaiwattana, Tom Ellis, Rodrigo Ledesma-Amaro

The Five-Primer Challenge: An inquiry-based laboratory module for synthetic biology

Suzie Hsu, Jay D. Huenemann, Vishwesh Kulkarni, Rodrigo Ledesma-Amaro, Robert C. Moseley, Nikita Mukhitov, Rashmi Tiwari, Shue Wang, Michael J. Smanski

TargeTron Technology Applicable in Solventogenic Clostridia: Revisiting 12 Years’ Advances

Zhiqiang Wen, Minrui Lu, Rodrigo Ledesma-Amaro, Li Qi, Mingjie Jin, Sheng Yang

Redirecting the lipid metabolism of the yeast Starmerella bombicola from glycolipid to fatty acid production

Sylwia Jezierska, Silke Claus, Rodrigo Ledesma-Amaro, Inge Van Bogaert

Rapid Assembly of gRNA Arrays via Modular Cloning in Yeast

Nicholas S. McCarty, W. M. Shaw, Tom Ellis, Rodrigo Ledesma-Amaro

One‐vector CRISPR/Cas9 genome engineering of the industrial fungus Ashbya gossypii

Alberto Jiménez, Gloria Muñoz‐Fernández, Rodrigo Ledesma-Amaro, Rubén M. Buey, José Luis Revuelta

INFLUENCE OF MICRO-ENVIRONMENT ON YEAST POPULATION DYNAMICS

Jean‐Yves Trosset, Sami Tliba, Ali El Ati, Hela Friha, Estelle Mogensen, Jawher Kahouli, Robin v. Lacombe, Ouerdia Arkoun, D. Sette, Rodrigo Ledesma-Amaro, Islam Boussaada

Improved n -Butanol Production from Clostridium cellulovorans by Integrated Metabolic and Evolutionary Engineering

Zhiqiang Wen, Rodrigo Ledesma-Amaro, Jianping Lin, Yu Jiang, Sheng Yang

Engineering a Bifunctional Phr60-Rap60-Spo0A Quorum-Sensing Molecular Switch for Dynamic Fine-Tuning of Menaquinone-7 Synthesis in Bacillus subtilis

Shixiu Cui, Xueqin Lv, Yaokang Wu, Jianghua Li, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu

Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica

Stefan Bruder, Eva J. Moldenhauer, Robert Denis Lemke, Rodrigo Ledesma-Amaro, Johannes Kabisch

Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in Bacillus subtilis

Yaokang Wu, Taichi Chen, Yanfeng Liu, Rongzhen Tian, Xueqin Lv, Jianghua Li, Guocheng Du, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

Creating an in vivo bifunctional gene expression circuit through an aptamer-based regulatory mechanism for dynamic metabolic engineering in Bacillus subtilis

Jieying Deng, Chunmei Chen, Yang Gu, Xueqin Lv, Yanfeng Liu, Jianghua Li, Rodrigo Ledesma-Amaro, Guocheng Du, Long Liu

Synthetic repetitive extragenic palindromic (REP) sequence as an efficient mRNA stabilizer for protein production and metabolic engineering in prokaryotic cells

Deng Chen, Xueqin Lv, Jianghua Li, Yanfeng Liu, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu

Synthetic redesign of central carbon and redox metabolism for high yield production of N-acetylglucosamine in Bacillus subtilis

Yang Gu, Xueqin Lv, Yanfeng Liu, Jianghua Li, Guocheng Du, Jian Chen, Rodrigo Ledesma-Amaro, Long Liu

Synthetic Biology Tools to Engineer Microbial Communities for Biotechnology

Nicholas S. McCarty, Rodrigo Ledesma-Amaro

Synthetic biology tools for engineering Yarrowia lipolytica

Macarena Larroudé, Tristan Rossignol, Jean‐Marc Nicaud, Rodrigo Ledesma-Amaro

Synthetic Biology to Improve the Production of Lipases and Esterases (Review)

Heber Gamboa‐Meléndez, Macarena Larroude, Young-Kyoung Park, Pauline Trebul, Jean‐Marc Nicaud, Rodrigo Ledesma-Amaro

Synergistic Rewiring of Carbon Metabolism and Redox Metabolism in Cytoplasm and Mitochondria of Aspergillus oryzae for Increased l-Malate Production

Jingjing Liu, Jianghua Li, Yanfeng Liu, Hyun‐Dong Shin, Rodrigo Ledesma-Amaro, Guocheng Du, Jian Chen, Long Liu

Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome

Benjamin A. Blount, Glen-Oliver F. Gowers, Jack C. H. Ho, Rodrigo Ledesma-Amaro, Dejana Jovicevic, Robert M. McKiernan, Ze‐Xiong Xie, Bing Li, Ying‐Jin Yuan, Tom Ellis

Pathway Grafting for Polyunsaturated Fatty Acids Production in Ashbya gossypii through Golden Gate Rapid Assembly

Rodrigo Ledesma-Amaro, Alberto Jiménez, José Luis Revuelta

Optimization of odd chain fatty acid production by Yarrowia lipolytica

Young-Kyoung Park, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jean‐Marc Nicaud

Genetic engineering of Ehrlich pathway modulates production of higher alcohols in engineered Yarrowia lipolytica

Ewelina Celińska, Monika Borkowska, W. Białas, Monika Kubiak, Paulina Korpys-Woźniak, Marta Archacka, Rodrigo Ledesma-Amaro, Jean‐Marc Nicaud

Formation of folates by microorganisms: towards the biotechnological production of this vitamin

José Luis Revuelta, Cristina Serrano-Amatriain, Rodrigo Ledesma-Amaro, Alberto Jiménez

Engineering Yarrowia lipolytica to enhance lipid production from lignocellulosic materials

Xochitl Niehus, Anne-Marie Crutz-Le Coq, Georgina Sandoval, Jean‐Marc Nicaud, Rodrigo Ledesma-Amaro

CRISPRi allows optimal temporal control of N-acetylglucosamine bioproduction by a dynamic coordination of glucose and xylose metabolism in Bacillus subtilis

Yaokang Wu, Taichi Chen, Yanfeng Liu, Xueqin Lv, Jianghua Li, Guocheng Du, Rodrigo Ledesma-Amaro, Long Liu

Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica

Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean‐Marc Nicaud, Christophe Leplat

The Engineering Potential of Rhodosporidium toruloides as a Workhorse for Biotechnological Applications

Young-Kyoung Park, Jean‐Marc Nicaud, Rodrigo Ledesma-Amaro

Sugar versus fat: elimination of glycogen storage improves lipid accumulation in Yarrowia lipolytica

Govindprasad Bhutada, Martin Kavšček, Rodrigo Ledesma-Amaro, Stéphane Thomas, Gerald N. Rechberger, Jean‐Marc Nicaud, Klaus Natter

Golden Gate Assembly system dedicated to complex pathway manipulation in Yarrowia lipolytica

Ewelina Celińska, Rodrigo Ledesma-Amaro, Macarena Larroudé, Tristan Rossignol, Cyrille Pauthenier, Jean‐Marc Nicaud

A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β‐carotene

Macarena Larroudé, Ewelina Celińska, Alexandre Back, T. Stephan, Jean‐Marc Nicaud, Rodrigo Ledesma-Amaro

Yarrowia lipolytica AAL genes are involved in peroxisomal fatty acid activation

Rémi Dulermo, Heber Gamboa‐Meléndez, Rodrigo Ledesma-Amaro, France Thévenieau, Jean‐Marc Nicaud

Synthetic biology and metabolic engineering to produce microbial oils: towards a sustainable source of fuels, chemicals and nutraceuticals

Rodrigo Ledesma-Amaro, José Luis Revuelta

Refactoring Ashbya gossypii for advanced biolipid production

Alberto Jiménez, David Díaz-Fernández, Patricia Lozano‐Martínez, Rodrigo Ledesma-Amaro, José Luis Revuelta

Overexpression of diacylglycerol acyltransferase inYarrowia lipolyticaaffects lipid body size, number and distribution

Peter Gajdoš, Rodrigo Ledesma-Amaro, Jean‐Marc Nicaud, Milan Čertík, Tristan Rossignol

Modulation of gluconeogenesis and lipid production in an engineered oleaginous Saccharomyces cerevisiae transformant

Yasushi Kamisaka, Kazuyoshi Kimura, Hiroshi Uemura, Rodrigo Ledesma-Amaro

Microbial biotechnology for the synthesis of (pro)vitamins, biopigments and antioxidants: challenges and opportunities

José Luis Revuelta, Rubén M. Buey, Rodrigo Ledesma-Amaro, Erick Vandamme

Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose

Rodrigo Ledesma-Amaro, Zbigniew Lazar, Magdalena Rakicka, Zhongpeng Guo, Florian Fouchard, Anne-Marie Crutz-Le Coq, Jean‐Marc Nicaud

Industrial Production of VitaminB2by Microbial Fermentation

José Luis Revuelta, Rodrigo Ledesma-Amaro, Alberto Jiménez

Folic Acid Production by Engineered Ashbya gossypii

Cristina Serrano-Amatriain, Rodrigo Ledesma-Amaro, Rubén López‐Nicolás, Gaspar Ros, Alberto Jiménez, José Luis Revuelta

Engineering Ashbya gossypii strains for de novo lipid production using industrial by‐products

Patricia Lozano‐Martínez, Rubén M. Buey, Rodrigo Ledesma-Amaro, Alberto Jiménez, José Luis Revuelta

Combining metabolic engineering and process optimization to improve production and secretion of fatty acids

Rodrigo Ledesma-Amaro, Rémi Dulermo, Xochitl Niehus, Jean‐Marc Nicaud

Bioproduction of riboflavin: a bright yellow history

José Luis Revuelta, Rodrigo Ledesma-Amaro, Patricia Lozano‐Martínez, David Díaz-Fernández, Rubén M. Buey, Alberto Jiménez

Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids

Rodrigo Ledesma-Amaro, Jean‐Marc Nicaud

Unraveling fatty acid transport and activation mechanisms in Yarrowia lipolytica

Rémi Dulermo, Heber Gamboa-Meléndez, Rodrigo Ledesma-Amaro, France Thévenieau, Jean‐Marc Nicaud

Structure of IMP dehydrogenase of Ashbya gossypii with IMP bound to the active site

Rubén M. Buey, Rodrigo Ledesma-Amaro, Mónica Balsera, José M. De Pereda, Julia Revuelta

Metabolic engineering of riboflavin production in Ashbya gossypii through pathway optimization

Rodrigo Ledesma-Amaro, Cristina Serrano-Amatriain, Alberto Jiménez, José Luis Revuelta

Increased riboflavin production by manipulation of inosine 5′-monophosphate dehydrogenase in Ashbya gossypii

Rubén M. Buey, Rodrigo Ledesma-Amaro, Mónica Balsera, José M. De Pereda, José Luis Revuelta

Increased production of inosine and guanosine by means of metabolic engineering of the purine pathway in Ashbya gossypii

Rodrigo Ledesma-Amaro, Rubén M. Buey, José Luis Revuelta

Guanine nucleotide binding to the Bateman domain mediates the allosteric inhibition of eukaryotic IMP dehydrogenases

Rubén M. Buey, Rodrigo Ledesma-Amaro, Adrián Velázquez‐Campoy, Mónica Balsera, Mónica Chagoyen, José M. De Pereda, José Luis Revuelta

EngineeringAshbya gossypiifor efficient biolipid production

Rodrigo Ledesma-Amaro, Patricia Lozano‐Martínez, Alberto Jiménez, José Luis Revuelta

Engineering Yarrowia lipolytica to produce biodiesel from raw starch

Rodrigo Ledesma-Amaro, Thierry Dulermo, Jean‐Marc Nicaud

Microbial oils: A customizable feedstock through metabolic engineering

Rodrigo Ledesma-Amaro

Strain Design of Ashbya gossypii for Single-Cell Oil Production

Rodrigo Ledesma-Amaro, M. Ángeles Santos, Alberto Jiménez, José Luis Revuelta

Genome scale metabolic modeling of the riboflavin overproducer Ashbya gossypii

Rodrigo Ledesma-Amaro, Eduard J. Kerkhoven, José Luis Revuelta, Jens Nielsen

Biotechnological production of feed nucleotides by microbial strain improvement

Rodrigo Ledesma-Amaro, Alberto Jiménez, M. Ángeles Santos, José Luis Revuelta